Although most species live in inshore and estuarine waters, 29 species spend their entire life cycles in freshwater. These species are found in disjunct tropical regions of South America (one species), Central Africa (three species) and Southeast Asia (25 species).
Natural defenses :
A pufferfish pressing its mouth against a camera's lens at Big Island of Hawaii
The puffer's unique and distinctive natural defenses help compensate for its slow locomotion. It moves by combining pectoral, dorsal, anal, and caudal fins. This makes it highly maneuverable, but very slow, and therefore a comparatively easy predation target. Its tail fin is mainly used as a rudder, but it can be used for a sudden evasive burst of speed that shows none of the care and precision of its usual movements. The puffer's excellent eyesight, combined with this speed burst, is the first and most important defense against predators. Its backup defense mechanism, used if successfully pursued, is to fill its extremely elastic stomach with water (or air when outside the water) until it is much larger and almost spherical in shape. Even if they are not visible when the puffer is not inflated, all puffers have pointed spines, so a hungry predator may suddenly find itself facing an unpalatable, pointy ball rather than a slow, tasty fish. Predators which do not heed this warning (or who are "lucky" enough to catch the puffer suddenly, before or during inflation) may die from choking, and predators that do manage to swallow the puffer may find their stomachs full of tetrodotoxin, making puffers an unpleasant, possibly lethal, choice of prey. This neurotoxin is found primarily in the ovaries and liver, although smaller amounts exist in the intestines and skin, as well as trace amounts in muscle. It does not always have a lethal effect on large predators, such as sharks, but it can kill humans.
Not all puffers are necessarily poisonous; Takifugu oblongus, for example, is a fugu puffer that is not poisonous, and toxin level varies wildly even in fish that are. A puffer's neurotoxin is not necessarily as toxic to other animals as it is to humans, and puffers are eaten routinely by some species of fish, such as lizardfish[3] and tiger sharks.Also, Japanese fish farmers have grown nonpoisonous puffers by controlling their diet.
Puffers are able to move their eyes independently, and many species can change the color or intensity of their patterns in response to environmental changes. In these respects, they are somewhat similar to the terrestrial chameleon. Although most puffers are drab, many have bright colors and distinctive markings, and make no attempt to hide from predators. This is likely an example of aposematism.
Reproduction :
Blackspotted puffer, Arothron nigropunctatus
Many marine puffers have a pelagic, or open-ocean, life stage. Spawning occurs after males slowly push females to the water surface or join females already present. The eggs are spherical and buoyant. Hatching occurs after roughly four days. The fry are tiny, but under magnification have a shape that is usually reminiscent of a pufferfish. They have a functional mouth and eyes, and must eat within a few days. Brackish water puffers may breed in bays in a similar manner to marine species, or may breed more similarly to the freshwater species, in cases where they have moved far enough upriver.
Reproduction in freshwater species varies quite a bit. The dwarf puffers court with males following females, possibly displaying the crests and keels unique to this subgroup of species. After the female accepts his advances, she will lead the male into plants, moss or another form of cover, where she can release eggs for fertilization. The male may help her by rubbing against her side. This has been observed in captivity, and they are the only commonly captive-spawned puffer species.
Target-group puffers have also been spawned in aquariums, and follow a similar courting behavior, minus the crest/keel display. However, eggs are laid on a flat piece of slate or other smooth hard material, to which they adhere. The male will guard them until they hatch, carefully blowing water over them regularly to keep the eggs healthy. His parenting is finished when the young hatch, and the fry are on their own.
Information on breeding of specific species is very limited. T. nigroviridis, the green-spotted puffer, has recently been artificially spawned under captive conditions. It is believed to spawn in bays in a similar manner to saltwater species, as their sperm were found to be motile only at full marine salinities, but actual wild breeding has never been observed.
In 2012, male pufferfish were documented carving large geometric, circular structures in the seabed sand in Amami Ōshima, Japan. The structures apparently serve to attract females and provide a safe place for them to lay their eggs.
